Part:BBa_K1369005:Design

AgrA; P2 promoter+GFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 289
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 312
  Illegal XhoI site found at 948
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 73
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1874
  Illegal SapI site found at 55
  

Design Notes

A double terminator sequence separates the AgrA sequence from the P2 promoter sequence.

Source

The AgrA response regulator and P2 promoter are endogenous to Staphylococcus aureus, which are involved in the AgrBDCA quorum sensing system.